The flanking sequences of the 3′ LTR were left unaltered by this strategy. Transfection of these chimeric vectors into 293T cells and harvest of supernatants 48 h later。
The molecular clone of feline foamy virus (pCF-7) and FeFAB cells for FeFAB assay were kind gifts from Dr Martin Löchelt, German Cancer Research Center, Germany.
FeFAB assay indicated that the culture supernatants collected from the infected and drug-pretreated cells contained infectious FFV virions,fefab which induce expression of the β-galactosidase in the ...
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